To protect the baculovirus from serum inactivation and to achieve a controlled release at the target site, the baculovirus nanohybrids were encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanospheres. The nanospheres were impregnated in a fibrin hydrogel and coated using layer-by-layer assembly on prefabricated stent surface which eventually acts as the scaffold to deliver the protected viral nanohybrids to the target site.
Thus, this new generation of gene-eluting stent device
can efficiently deliver angiogenic vascular genes (e.g. vascular endothelial growth factor, Vegf) to the affected site and induce favorable therapeutic effect in the local vasculature
to promote wound healing.